Fig. 4.
The siRNA-mediated knockdown of post-ischemic Egr1 expression decreased infarction and inflammatory gene expression in adult rats subjected to transient MCAO. Egr1 siRNA depleted the Egr1 mRNA levels (a) as well as Egr1 protein levels (b) in the post-ischemic brain. Panel c shows Cresyl violet-stained serial sections from representative rats subjected to transient MCAO following treatment with Egr1 and control siRNA. Egr1 siRNA treatment also significantly curtailed the post-ischemic induction of the mRNA expression of cytokines, chemokines and ICAM1 (d). The values in the histograms are mean ± SD of n = 4/group. In panel a, ap < 0.05 compared to the respective sham and bp < 0.05 compared to the control siRNA group and in panel d, *p < 0.05 compared to the control siRNA group by ANOVA followed by Tukey-Kramer multiple comparisons post-test.