Table 2.

Real-time PCR analysis of inflammatory mRNA expression in the Egr1+/+ and Egr1-/- mice subjected to transient MCAO

		6 h reperfusion		1 day reperfusion
Transcript	GenBank#	Egr1+/+	Egr1-/-	Egr1+/+	Egr1-/-
The values are mean ± SD of fold differences of 16 cross-comparisons between four sham and four ischemic animals in each case. Total RNA was extracted from the ipsilateral cortex of each mouse. Real-time PCR was conducted in duplicate with each cDNA sample for each transcript.
IL6	NM_031168	17.1 ± 2.4	5.2 ± 1.4*	56.7 ± 6.1	6.4 ± 1.1*
IL1β	NM_008361	5.7 ± 1.1	1.8 ± 0.7*	8.9 ± 2.0	2.1 ± 0.6*
MIP1α	NM_011337	22.0 ± 3.3	8.2 ± 1.6*	18.8 ± 2.5	6.3 ± 1.4*
MCP1	AF065933	15.6 ± 2.2	4.1 ± 0.9*	19.4 ± 2.9	5.3 ± 1.0*
ICAM1	BC008626	7.7 ± 1.5	2.1 ± 0.5*	9.6 ± 2.0	1.7 ±0.3*
CC-C3	NM_009778	23.7 ± 3.1	4.5 ± 0.4*	34.8 ± 5.4	5.7 ± 1.5*
HSP70	AW763765	7.2 ± 1.3	6.4 ± 1.2	12.1 ± 2.0	10.6 ± 1.5
HSP27	U03561	4.5 ± 0.9	5.2 ± 1.3	5.1 ± 0.7	5.8 ± 1.1
ODC	NM_013614	4.8 ± 1.4	4.1 ± 1.0	2.3 ± 0.4	1.8 ± 0.4
18S rRNA	M11188	NC	NC	NC	NC
GAPDH	NM_008084	NC	NC	NC	NC

^*p < 0.05 compared to Egr1+/+ mice by one-way anova followed by Tukey-Kramer multiple comparisons post-test.