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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Cell Signal. 2008 Jul 2;20(10):1900–1910. doi: 10.1016/j.cellsig.2008.06.019

Figure 2. Localization of αs polybasic mutants.

Figure 2

HEK293 cells were transfected in 6-well plates containing coverslips with 0.4 µg pcDNA3 along with 0.6 µg αspcDNA3 (A), αs5QpcDNA3 (B), or αs4QpcDNA3 (C). 40 hours after transfection cells were fixed with 3.7% formaldehyde and visualized as described under “Experimental Procedures.” D, HEK293 cells were transfected in 6-cm plates with 1.2 µg pcDNA3 along with 1.8 µg pcDNA3 containing the indicated α subunit. Approximately 40 hours after transfection cells were lysed in hypotonic lysis buffer and soluble and particulate cell fractions were separated as described under “Experimental Procedures.” 2.5% of each fraction was resolved on 12% SDS-PAGE and the α subunits were visualized by immunoblotting with the 12CA5 antibody.