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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Cell Signal. 2008 Jul 2;20(10):1900–1910. doi: 10.1016/j.cellsig.2008.06.019

Figure 5. Localization of αq7E and αq9Q2.

Figure 5

HEK293 cells were transfected in 6-well plate containing coverslips with 0.4 µg pcDNA3 along with 0.6 µg pcDNA3 containing αq7Q (A), αq9Q (B), αq7E (C), or αq9Q2 (D). 40 hours after transfection cells were fixed and stained with 12CA5 antibody as described under “Experimental Procedures.” E, HEK293 cells were transfected with 0.6 µg pcDNA3 along with 2.4 µg pcDNA3 containing an αq mutant. 40 hours after transfection cells were lysed in hypotonic lysis buffer and soluble and particulate cell fractions were separated as described under “Experimental Procedures.”