Skip to main content
. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Cell Signal. 2008 Jul 2;20(10):1900–1910. doi: 10.1016/j.cellsig.2008.06.019

Figure 6. Localization of myristoylated polybasic mutants.

Figure 6

HEK293 cells were transfected in 6-well plates containing coverslips with 0.4 µg pcDNA3 along with 0.6 µg pcDNA3 containing myr-αs5QpcDNA3 (A), myr-αq9QpcDNA3 (B), or myr-αq3EnpcDNA3 (C). 40 hours after transfection cells were fixed with 3.7% formaldehyde and visualized as described under “Experimental Procedures.” For fractionations, HEK293 cells were transfected in 6-cm plates with 1.2 µg pcDNA3 along with 1.8 µg pcDNA3 containing the indicated αs construct (D) or αq construct (E). 40 hours after transfection cells were lysed and fractionated as described under “Experimental Procedures.” Note that myr-αq9Q and myr-αq3En migrate slightly faster than αq9Q and αq3En, respectively. This is likely due to deletion of amino acids 1–6 in the myristoylated forms [37].