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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Cell Signal. 2008 Jul 2;20(10):1900–1910. doi: 10.1016/j.cellsig.2008.06.019

Figure 7. Effects of coexpressing β1γ2 on the localization of polybasic mutants.

Figure 7

HEK293 cells were transfected in 6-well plates containing coverslips with 0.6 µg of either αs5Q pcDNA3 (A–C), αq9QpcDNA3 (D–F) or αq3EnpcDNA3 (G–I). α subunits were transfected along with either 0.4µg pcDNA3 (A, D, G) or 0.3 µg mycHis-β1pcDNA3 and 0.1 µg γ2pcDNA3 (B, C, E, F, H, I). 40 hours after transfection cells were fixed and stained with 12CA5 antibody (A, B, D, E, G, H) and anti-γ2 antibody (C, F, I) as described under “Experimental Procedures.” J, K, HEK293 cells were transfected with 1.8 µg pcDNA3 containing the indicated α subunit along with either 1.2 µg pcDNA3 or 0.9 µg mycHis-β1pcDNA3 plus 0.3 µg γ2pcDNA3. 40 hours after transfection cells were lysed and fractionated as described under “Experimental Procedures.” A portion of each fraction was resolved by SDS-PAGE and immunoblotted with either the 12CA5 antibody (upper panels) or 9E10 anti-myc antibody (lower panels).