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. Author manuscript; available in PMC: 2009 Aug 26.
Published in final edited form as: Biochemistry. 2008 Aug 2;47(34):8840–8854. doi: 10.1021/bi800301b

Figure 3. Kvβ2-catalyzed reduction of phospholipid aldehyde.

Figure 3

Electrospray mass spectra of 1-palmitoyl-2-oxovaleroyl phosphatidyl choline (POVPC) incubated without (A) and with (B) Kvβ2. The formation of the reduced product - 1-palmitoyl-2-oxovaleroyl phosphatidyl choline (PHVPC) is evident by the appearance of a new ion with m/z = 596.4. For reduction, POVPC (80 μM) was incubated without or with Kvβ2 (35 μM) and NADPH (0.15 mM) for 180 min at 37°C. The 2Da increase in m/z was assigned the reduction of the aldehyde at the sn-2 position to an alcohol (as indicated).