Table 1.
Plasma cytokine concentration after i.p. injection of PGN alone, or after PGN followed by LPS challenge.
| Pretreatment | Challenge | IFNγ
(pg/ml) |
IL-10
(pg/ml) |
TNFα
(pg/ml) |
|---|---|---|---|---|
| PGN – 6hrs1 | None | ND | 418 ± 135 * | 123 ± 44 * |
| Saline – 6hrs | None | ND | ND | ND |
| PGN – 48hrs2 | None | ND | ND | ND |
| Saline – 48hrs | None | ND | ND | ND |
| PGN – 48hrs3 | LPS | 22 ± 17 * | 1850 ± 473 * | 103 ± 36 |
| Saline – 48hrs | LPS | 140 ± 76 | 594 ± 154 | 148 ± 49 |
1
PGN (or saline) was injected and blood samples collected either 6 or 48 hours later without further challenge.
2
PGN (or saline) was injected and blood samples collected either 6 or 48 hours later without further challenge.
3
In the third group, LPS was injected 48 hours after PGN pretreatment and blood collected 6 hours after the LPS challenge. Data shown are Mean ± SEM. ND = Not detectable. Lower limit of detection for IFNγ assay was 8 pg/ml; 30pg/ml for the IL-10 assay; and 8 pg/ml for the TNFα assay.
*
= p<0.05 vs. respective saline control, N = 5 per group.