Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2009 Sep 15.

Published in final edited form as: Free Radic Biol Med. 2008 Jun 30;45(6):929–938. doi: 10.1016/j.freeradbiomed.2008.06.024

Fig. 1 — A) RBMEC were preloaded with DCFH-DA or C-369 (non-oxidizable control probe) for 30 min. Cells were then irradiated with 1–10 Gy γ-rays, and the relative fluorescence was measured 1 h postirradiation. Mean ± SD; n = 3; *p<0.05 and **p<0.01 vs. sham-irradiated control. B) RBMEC were pretreated with 5 mM NAC (30 min), 10 µM DPI, or 10 µM apocynin (1 h) and relative fluorescence was measured 1 h postirradiation. Mean ± SD; n=3; ### p< 0.001 vs. sham-irradiated control and ***p<0.001 vs. 10 Gy. C) RBMEC were transfected with 0.8 µg p22^phox antisense oligonucleotides. Left panel: 24 h post-transfection, total RNA was collected and analyzed for p22^phox gene expression by Northern blot. Right panel: relative fluorescence was measured 1 h postirradiation. Mean ± SD; n = 3; ### p< 0.001 vs. sham-irradiated control and ***p<0.001 vs. 10 Gy. D) RBMEC were pretreated with 2µM rotenone, 50 µM oxypurinol, or 1 mM L-NAME for 1 h prior to irradiation, and the relative fluorescence was measured 1 h postirradiation. Mean ± SD; n = 3; ## p< 0.01 vs. sham-irradiated control and ***p<0.001 vs. 10 Gy.

Fig. 1 — A) RBMEC were preloaded with DCFH-DA or C-369 (non-oxidizable control probe) for 30 min. Cells were then irradiated with 1–10 Gy γ-rays, and the relative fluorescence was measured 1 h postirradiation. Mean ± SD; n = 3; *p<0.05 and **p<0.01 vs. sham-irradiated control. B) RBMEC were pretreated with 5 mM NAC (30 min), 10 µM DPI, or 10 µM apocynin (1 h) and relative fluorescence was measured 1 h postirradiation. Mean ± SD; n=3; ### p< 0.001 vs. sham-irradiated control and ***p<0.001 vs. 10 Gy. C) RBMEC were transfected with 0.8 µg p22^phox antisense oligonucleotides. Left panel: 24 h post-transfection, total RNA was collected and analyzed for p22^phox gene expression by Northern blot. Right panel: relative fluorescence was measured 1 h postirradiation. Mean ± SD; n = 3; ### p< 0.001 vs. sham-irradiated control and ***p<0.001 vs. 10 Gy. D) RBMEC were pretreated with 2µM rotenone, 50 µM oxypurinol, or 1 mM L-NAME for 1 h prior to irradiation, and the relative fluorescence was measured 1 h postirradiation. Mean ± SD; n = 3; ## p< 0.01 vs. sham-irradiated control and ***p<0.001 vs. 10 Gy.