Skip to main content
. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: Free Radic Biol Med. 2008 Jun 30;45(6):929–938. doi: 10.1016/j.freeradbiomed.2008.06.024

Fig. 6. Ionizing radiation can modulate the expression of NAPDH oxidase components in RBMEC.

Fig. 6

Fig. 6

Fig. 6

Fig. 6

A and B) RBMEC were irradiated with 10 Gy γ-rays, and total RNA was collected 1–8 h postirradiation. mRNA expression of Nox4 (A) and p22phox (B) was assessed by real-time PCR. Values are expressed relative to β-actin. Mean ± SD; n = 3; *p<0.05 and **p<0.01 vs. sham-irradiated control. C) Total cell lysates were collected 3–24 h postirradiation, separated by SDS-PAGE, and probed for p22phox. β-actin served as a loading control. Densitometric analysis is shown. Mean ± SD; n = 3; *p<0.05 and vs. sham-irradiated control. D) Total cell lysates were collected 2–24 h postirradiation, separated by SDS-PAGE, and probed for p47phox. Densitometric analysis is shown. Mean ± SD; n = 3; *p<0.05 vs. sham-irradiated control.