Fig. 4.

Trop2^hi LSC cells give rise to basal, luminal, and neuroendocrine cells in vivo. (A) A total of 3 × 10³ Trop2^hi LSC or Trop2^lo LSC cells from β-actin DsRed mice, 10⁵ total prostate cells from β-actin GFP mice, and 2 × 10⁵ UGSM cells were combined in Matrigel and injected s.c. into SCID mice. Grafts were harvested after 8 weeks, and overview images were taken by using transillumination (TI) and DsRed or GFP fluorescence. (B) Tissue sections from regenerated tissue were stained for antibodies against cytokeratin 5 (K5), cytokeratin 8 (K8), androgen receptor (AR), p63, and synaptophysin. Sections were counterstained with DAPI (blue) nuclear stain. Sections from 8- to 12-week-old mice were stained for comparison of neuroendocrine cell morphology and marker expression pattern (synaptophysin). (C) In tissue sections from regenerated tissue, DsRed and GFP fluorescence (13 and 16) were visualized before antigen retrieval caused by a loss of signal after treatment. The remaining images demonstrate synaptophysin staining and DAPI nuclear counterstain after antigen retrieval.