Fig. 5.

Growth of wild-type and ICP0 null HSV in the presence and absence of BAG3 and/or PML. (A) MeWo, siPML, siBAG3, and siPML/siBAG3 cells were fixed and BAG (red) and PML (green) were visualized by indirect immunofluorescence microscopy. Images were captured with a 100× objective and analyzed by volume deconvolution. The individual separated channels are presented as Fig. S5. (B) MeWo, siPML, siBAG3, and siPML/siBAG3 cells were lysed and total cell lysates were analyzed by SDS/PAGE. The levels of PML, BAG3, and GAPDH were monitored by Western blot analysis. (C) MeWo (solid blue), siPML (solid red), siBAG3 (dashed blue), and siPML/siBAG3 (dashed red) with HSV (squares) or ICP0 null virus (triangles) at an moi of 0.1. At the indicated times after infection viruses were harvested and titrated on L7 cells.