Table 1.
Primer sequences (5′ to 3′) used for quantitative RT-PCR
| Gene |
Primer Sequence |
Accession No. | |
|---|---|---|---|
| Forward | Reverse | ||
| Primary microRNAs | |||
| pri-miR-1-1 | GGCGTCCCGGGGTC | TGTGGAGTGCCCCTCAGT | |
| pri-miR-1-2 | TTTCATGTTTTTACAGCTAACAACTTAGTAA | TAGCTCAGTTTAATTCTCCCTTGG | |
| pri-miR-133a-1 | TAACTGTTTTGGATTCCAAACTAGCA | ACAAATGAAAACGTTGGTTGTCC | |
| pri-miR-133a-2 | CCGACGTCGCTGTTCCT | TGCGGGACCTGCGG | |
| pri-miR-206 | CTTCTGAGATGCGGGCTG | CTCCGGGTGGTACCCTGG | |
| Drosha | ACTGTGGCTGTTTATTTCAAGGG | ATTTTTCAAGCGCATCCATTG | NC000005 |
| Dicer | ATCCCATGATGCGGCC | CTAAATTTGGCAGTTTCTGGTTCC | NC000014 |
| Exportin-5 | GCGCCGAAAGGACCA | AGGGAAGATTCTTAATGTGAACTTCTT | AC000049 |
Primary microRNA sequences were determined from the miRBase sequence data base (http://microrna.sanger.ac.uk/sequences/index.shtml).