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VP exocytotic effect is specific. A: application of methyl-β-cyclodextrin (mβCD, 10 mM), an inhibitor of endocytosis, leads to accumulation of AQP2 at the cell surface (15 min incubation). B: mβCD did not lead to a significant increase in exocytosis as measured by ssYFP fluorimetry assay in LLC-ssYFP or LLC-AQP2-ssYFP cells. Relative fluorescence values were background subtracted and calculated relative to LLC-AQP2-ssYFP at 15 min.
