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. Author manuscript; available in PMC: 2009 Aug 25.
Published in final edited form as: Chem Biol. 2008 Aug 25;15(8):765–770. doi: 10.1016/j.chembiol.2008.07.010

Figure 4.

Figure 4

(A) In vitro CoASH formation from acetyl-CoA and phosphonopyruvate or phosphoenolpyruvate depends on the presence of FrbC or FrbC + FrbD respectively. (B) Selected ion chromatogram for 2-phosphonomethylmalate +/− 1 ppm from an injection of reaction mixture (20 µL) with summed mass spectrum (inset). (C) High resolution MS2 spectrum for the precursor ion at negative m/z 227 showing the predictable losses of water and the added acetyl group.