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. Author manuscript; available in PMC: 2009 Aug 29.
Published in final edited form as: J Mol Biol. 2008 Jun 18;381(2):261–275. doi: 10.1016/j.jmb.2008.06.035

Figure 7. Effect of the spacer sequence separating RE1 and RE2 in transcription activation of rapF.

Figure 7

Cultures containing PrapF-lacZ fusions were grown in defined minimal medium and aliquots taken throughout growth for determination of β-galactosidase specific activity. The time point containing the maximal β-galactosidase activity which typically peaks at OD600 1–1.5 is shown. The wild type RE1-RE2 spacer is 5’-TGTA and defined as 100%. Substitutions in position 1 of the spacer: KG331 (T-G); KG282 (T-A); and KG283 (T-C). Position 2: KG284 (G-A); KG285 (G-T); and KG305 (G-C). Position 3: KG311 (T-G); KG312 (T-A); and KG286 (T-C). Position 4: KG325 (A-G); KG310 (A-T); and KG287 (A-C). The data are an average of three independent experiments with standard deviation shown.