TABLE 2.
Single nucleotide polymorphism genotyping assay information
| Reporter | |||
|---|---|---|---|
| SNP | VIC* | FAM† | Context sequence‡ |
| −8202A/G | A | G | 5′GCCCAGCCCAGCCTGCAGCCCTGGG[A/G]CTCTGCAGCAGCACAGTCAGAAATG 3′ |
| IVS4+3G/T | G | T | 5′GTGGTCCCTGGGCAAGGGCGTCGGT[G/T]AGATTCTGAGTCCTCCTGGCCCCTG 3′ |
| 2003A/G (Q668R) | A | G | 5′GACACGCACGACGTCTTCCAGTACC[A/G]AGGTGAGGGCTGAGGAGGATCCCTT 3′ |
SNP, Single nucleotide polymorphiosm.
VIC dye used to label the TaqMan probe that detects the allele 1 sequence.
FAM dye used to label the TaqMan probe that detects the allele 2 sequence.
Twenty-five nucleotides on both sides of the SNP site, which is indicated in brackets (ie, NNN[A1/A2]NNN, where A1 = allele 1 and A2 = allele 2).