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Summary of the effects of extracellular Ca2+ on GSH and ROS accumulation in isolated myocytes and intact heart. Removal of extracellular Ca2+ (nominally Ca2+ free) decreases GSH (upper panels) and increases ROS levels (lower panels) in intact hearts (a) and isolated cardiomyocytes (b) when compared with their respective controls (0.5mM Ca2+ in intact hearts and 1mM Ca2+ in isolated cardiomyocytes).
