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Localization of superoxide and H2O2 production in perfused whole heart during ischemia/reperfusion. Double labeling of intact hearts with the mitochondrial superoxide indicator (MitoSOX) and CM-DCF indicate that both ROS contribute to ischemia-reperfusion-related oxidative stress and cellular heterogeneity, consistent with the mitochondrial respiratory chain being the source of the oxidative stress. Scale bar equals 40 microns.
