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Enhanced GSH levels upon raising perfusate Ca2+ in the intact heart. a) An intact heart loaded with TMRE and MCB was perfused with nominally Ca2+-free buffer under normoxic conditions (left panels). Subsequent perfusion with buffer containing 100μM Ca2+ increased GSB fluorescence (right panels). b) GSB fluorescence increase following increase in Ca2+ in the perfusate. Scale bar in a equals 20 microns
