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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Dev Cell. 2008 Aug;15(2):272–284. doi: 10.1016/j.devcel.2008.07.008

Figure 3. miR-126 regulates vascular integrity and lumen maintenance in vivo.

Figure 3

(A) miR-126 and miR-126* enrichment (qRT-PCR) in GFP+ endothelial cells from 72 hpf Tg(flk1:GFP)s843 zebrafish compared to GFP− cells. (B) Relative levels of miR-126 and miR-126* in 72 hpf zebrafish embryos. (C) miR-126 expression monitored by qRT-PCR during zebrafish development. (D) Levels of miR-126/126* or egfl7 (across intron containing miR-126) quantified by qRT-PCR in 72 hpf zebrafish injected with miR-126 MOs relative to control. (E) Lateral views of control and miR-126 MO-injected Tg(flk1:GFP)s843; Tg(gata1:dsRed)sd2 zebrafish (72 hpf). Brightfield microscopy (top) revealed no major changes in gross morphology, while flk1:GFP showed normal blood vessel patterning (middle). Presence of blood cells (gata1:dsRed) in the intersomitic vessels (isv), dorsal aorta (da) and primary cardinal vein (pcv) was greatly reduced in morphants (bottom). y, yolksac; h, head; ht, heart. (F) miR-126 morphants had normal vessel patterning (flk1:GFP), but developed cranial hemorrhages (gata1:dsRed; arrow) in the head. baa, branchial arch arteries. (G) Ventral view of baa suggested smaller luminal size (indicated by arrow) in miR-126 morphants. (H) Transverse section of control or miR-126 MO-treated zebrafish revealed that the da and pcv of morphants had a smaller lumen than controls; higher magnification (right panels) of boxed area shows collapsed da and small pcv in morphants. flk1:GFP, green; ZO-1, an epithelial marker, red.