Verification of genes differentially expressed during SH. A, left: temporal expression pattern of the Edn1 gene (residing in cluster 1) fitted by LRSA. Details of the plot can be found in Fig. 4 legend. Right: expression level of Edn1 (relative to its expression in RA control rats) measured by qRT-PCR. Data for rats in response to RA or to IH or SH for 1, 3, 7, 14, or 30 days are shown. B–H: verification of other genes differentially expressed in SH: Bmp2, Mmp9, Cd8b, Cyp26b1, Cacna1c, Bmpr2, and Smad1. Data are means ± SE. Significant differences: *P < 0.05 compared with RA control rats.