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Translocation of p65-containing NF-κB to the nucleus in response to IL-1β. Cells were treated with control or 10 ng/ml IL-1β for up to 120 min, and nuclear extracts were assayed for p65-containing NF-κB by ELISA. A: response in primary cells grown in ALI culture for 3 wk. Values are means ± SE (n = 3 collected from 3 normal donors). B: response in the 16HBE14o− cell line. Values are means ± SE (n = 6). *P = 0.05; † P = 0.01 vs. time 0.
