Fig. 5.

Specific knockdown of Ror2 expression abolished the antiproliferative effects of AEA and rWnt 5a. Mz-ChA-1 cells were stably transfected with Ror2 shRNA vectors. The expression of Ror2 was assessed in the parental cell line (Mz-ChA-1), the mock-transfected cell line (Mz-puro neg), and the cell line containing the Ror2 shRNA (Mz-Ror2) by real-time PCR and immunoblotting (A). Data are expressed as averages ± SE after correction for GAPDH expression. The effect of AEA and rWnt 5a treatment on cell proliferation was assessed by MTS assays (B). The 3 cell lines were treated with AEA (10 μM) or rWnt 5a (50 ng/ml) for 48 h, and data are expressed as fold change in proliferation (average ± SE, n = 7); *P < 0.05 compared with basal treatment within each cell line.