Fig. 7.

Coimmunoprecipitation of AMPK and protein phosphatase 2A (PP2A). AMPK was immunoprecipitated (IP) from H4IIEC3 cells, and AMPK or PP2A was detected in Western blots (WB). A: Western blot analysis confirmed the presence of AMPK in the immunoprecipitates. B: AMPK and PP2A can be coimmunoprecipitated although we did not see an increased amount of PP2A associated with AMPK after ethanol treatment (see discussion in text). The blot is a representative of 5 blots from 5 independent experiments.