Fig. 6.

Effects of selected protein kinase inhibitors on carbachol-stimulated D52 S¹³⁶ phosphorylation in HEK293 cells expressing HA-tagged D52. S¹³⁶ phosphorylation was analyzed as described in Fig. 5. A: cells were incubated with predicted maximal concentrations of the indicated inhibitors then stimulated with 10 μM carbachol for 1 min. Only staurosporine and W7 significantly inhibited S¹³⁶ phosphorylation. **P < 0.001, N = 3–6 experiments. B: dose-dependent effects of staurosporine on carbachol-stimulated S¹³⁶ phosphorylation. The apparent IC₅₀ was 3.3 nM, N = 4. C: dose-dependent effects of W7 on carbachol-stimulated S¹³⁶ phosphorylation. The apparent IC₅₀ was 38 μM, N = 4.