Table 2.
Effect of intracellular regulatory pathway modulators/inhibitors on 14C-ascorbic acid uptake in HepG2 cells
| Intracellular Regulatory Pathway | Specific Pathway Modulator | 14C-Ascorbic Acid Uptake pmol·mg−1·3 min−1 |
|---|---|---|
| Control | None | 700±15 |
| PKC | PMA | |
| 1 μM | 352±25 | |
| 4-α-PMA | ||
| 1 μM | 654±24 | |
| PKA | 8-BrcAMP | |
| 1 mM | 406±35 | |
| PTK | Genistein | |
| 50 μM | 415±13 | |
| 100 μM | 335±13 | |
| Tyrphostin A25 | ||
| 50 μM | 559±16 | |
| 100 μM | 355±7 | |
| Ca2+/CaM | CaM | |
| 10 μM | 303±8 | |
| 25 μM | 92±10 | |
| 50 μM | 78±11 | |
| TFP | ||
| 50 μM | 300±19 | |
| 100 μM | 64±6 | |
| W13 | ||
| 100 μM | 435±4 | |
| NO | SNAP | |
| 0.5 mM | 660±24 | |
| SNP | ||
| 0.5 mM | 650±24 | |
| 8-BrcGMP | ||
| 0.5 mM | 600±30 |
PKC, protein kinase C; PMA, phorbol 12-myristate 13-acetate; PKA, protein kinase A; 8-BrcAMP, 8-bromo cyclic-AMP; PTK, protein tyrosine kinase; Ca2+/CaM, calcium calmodulin; CaM, calmidazolium; TFP, trifluroperazine; W13, N-(4-aminobutyl)-5-chloro-2-naphthalenesulfonamide; NO, nitric oxide; SNAP, S-Nitrose-N-acetylpenicillamine; SNP sodium nitroprusside; 8-BrcGMP, 8-bromo cyclic-GMP. For all significant differences, P < 0.01.