Table 2.
μCT analysis of trabecular bone parameters in femur (mean ± SEM). N = 7-8 mice per group
| Wild type | Haploinsufficient | Null | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Parameter | 10 wk | 14 wk Vehicle | 14 wk PTH | 10 wk | 14 wk vehicle | 14 wk PTH | 10 wk | 14 wk vehicle | 14 wk PTH |
| Bone volume fraction (BV/TV, %) | 9.0 ±0.5 | 8.7 ± 0.7 | 17.0 ± 2.2 b | 11.4 ± 0.01 | 8.3 ± 0.3 a | 17.6 ± 1.9 b | 9.5 ± 1.0 | 7.6 ± 0.7 | 11.6 ± 1.1 b |
| Trabecular thickness (μm) | 44.6 ± 1.1 | 46.9 ± 1.0 | 56.5 ± 2.1 b | 45.2 ± 1.84 | 49.3 ± 1.9 | 59.5 ± 1.9 b | 43.0 ± 0.6 | 48.4 ± 1.5 | 56.2 ± 1.7 b |
| Trabecular number (per mm) | 4.30 ± 0.08 | 4.01 ± 0.06 a | 4.51 ± 0.16 b | 4.66 ± 0.13 | 3.95 ± 0.08 a | 4.59 ± 0.15 b | 4.24 ± 0.08 | 3.53 ± 0.16 c, a | 3.41 ± 0.08 |
| Trabecular spacing (μm) | 233 ± 5 | 247 ± 4 | 220 ± 7 | 214 ± 7 | 253 ± 5 | 223 ± 8 | 237 ± 5 | 289 ± 15 a | 305 ± 7 |
| Connectivity density (per mm3) | 79 ± 5 | 81 ± 7 | 183 ± 26 b | 114 ± 15 | 58 ± 6 c, a | 162 ± 16 b | 96 ± 14 | 52 ± 6c | 133 ± 26 b |
a
significantly different from corresponding 10 week, p ≤ 0.02
b
significantly different from corresponding vehicle treated, p ≤ 0.02
c
significantly different from vehicle treated wild type, p ≤ 0.03