Fig. 3.

Podocyte loss during nephrogenesis causes extensive developmental and pathological changes in the glomeruli. A, C, E, and G: newborn control mice. B, D, F, and H–J: newborn mutants. These are all hematoxylin- and eosin-stained paraffin sections. The overall structural division is preserved in the mutant kidney with nephrogenic zone, cortex, and outer and inner medulla (B), although a number of anomalies were noted and will be described in this and other figures in more detail. Renal vesicles and S-shaped bodies are formed normally in the mutants (C and F). However, the mutant glomeruli have a variety of severe problems. Proteinaceous material was present in Bowman's space (BS) of some glomeruli and tubular lumens (* in H). Other glomeruli have red blood cells in Bowman's space (arrow in I) and the tubular lumen (* in I). Arrow in J points to a disintegrating glomerulus with apparent apoptotic bodies. Arrowhead (I) points to apparent extravasation of red blood cells in the interstitium adjacent to the glomerulus.