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. 2008 Sep 17;295(6):F1658–F1665. doi: 10.1152/ajprenal.00022.2008

Fig. 3.

Fig. 3.

A: immunoblot using chicken antibody to ROMK in homogenates of dog (n = 2) bladder smooth muscle (and serosal) tissue and scraped epithelial cells and rat (n = 2) bladder muscle (and serosal) tissue and scraped epithelial cells. B: ROMK signal was ablated when ROMK antibody was preabsorbed with its immunizing peptide. C: immunoblot using chicken antibody to ROMK is homogenates of rat whole bladder, bladder muscle, and bladder epithelia. D: immmunoblot using antibody to calponin, a marker of smooth muscle cells, in the same nitrocellulose membrane used in C. Calponin was not identified in the epithelial cell scrapings. BW, whole bladder; BM, bladder muscle; BE, bladder epithelial cells.