Fig. 5.

Expression of ROMK mRNA using RT-PCR in homogenates of rat aorta, whole bladder, renal cortex, heart, liver, large intestine, small intestine, renal outer medulla, quadriceps muscle, ureter, and fibroblasts from cell culture. A single expected 474-bp was detected in all tissues except the fibroblast cells. In contrast, RT-PCR for the mRNA encoding the gene for the ubiquitous protein glyceraldehyde-3-phosphate dehydrogenase found the expected 309-bp product in all tissues, including fibroblasts (data not shown). No PCR product was obtained when RT was omitted.