Fig. 2.
Parameters of NaCl metabolism under water load (w) and water load + amiloride (w+amil) treatment from a 2-h experimental period. A and E: plasma Na+ (P
) and Cl− (P
) concentrations. B and F: urinary Na+ (U
) and Cl− (U
) concentrations. C and G: Na+ (FE
) and Cl− (FE
) fractional excretions. D and H: urinary Na+ and Cl− excretion, calculated for 24 h. Mice with a defect in CLDN16 develop hyponatremia under amiloride treatment. Increase in FE
and FE
after amiloride treatment is doubled in CLDN16 KD compared with WT mice. Dashed lines indicate values in WT under control conditions. Data are means ± SE; n = 6–8/group. *P < 0.05, WT vs. KD; §P < 0.05, w vs. w+amil. BW, body weight.