Fig. 3.

Renal function parameters under water load and water load + amiloride treatment from a 2-h experimental period (same experiment as in Fig. 2). A: urinary flow rate. B: plasma creatinine concentration. C: urine creatinine concentration. D: 2-h creatinine clearance. CLDN16 KD mice show limitations in water and electrolyte handling under amiloride treatment. Comparison of basic kidney parameters reveals that CLDN16 KD fails to further increase urinary flow under amiloride. Plasma creatinine was significantly higher and further increased under amiloride injection as a measure for impairment of glomerular filtration rate (GFR). Dashed lines indicate values in WT mice under control conditions. Data are means ± SE; n = 6–8/group. *P < 0.05, WT vs. KD; §P < 0.05, w vs. w+amil.