FIG. 9.

Axotomy induces LTH-M of isolated sensory neurons in culture medium containing 0.1% DMSO. A: photomicrographs of a Control neuron on Day 1 and the same neuron on Day 2. The neuron exhibited a modest increase in outgrowth over the 24-h period. B: photomicrographs of an Axotomized neuron on Day 1, prior to axotomy (left), the same neuron immediately after axotomy on Day 1 (0:00 h, middle), and the neuron 24 h later on Day 2 (right). Axotomy produced a more dramatic increase in outgrowth than was observed in the Control neuron (A). C: the change in the number of branch points from Day 1 to Day 2 for the Axotomized and Control groups. The difference between the 2 groups was statistically significant, as indicated by the asterisk. This pattern of results was like that observed in earlier experiments using culture medium without DMSO (Bedi and Glanzman 2001; Bedi et al. 1998).