FIG. 6.
The effect of BDNF on mEPSC frequency required presynaptic trkB receptors and did not involve retrograde signaling. A: individual example time courses for the effect of BDNF (0.8 nM) with vehicle, bath-applied K-252a, or postsynaptic K-252a. B: group data for effect of BDNF on mEPSC frequency in the presence of vehicle, bath-applied K-252a (200 nM), or K-252b (400 nM), or intracellular K-252a (200 nM). C: group data for effect of BDNF in the presence of the mGluR I/II antagonist MCPG (500 μM), the nitric oxide synthase inhibitor L-NAME (500 μM), the GABAB receptor antagonist CGP 35348 (CGP; 100 μM), or the adenosine A2A antagonist ZM 241385 (ZM, 50 nM). Recordings were from layer 5 PNs and were conducted in the presence of 2 mM extracellular Mg2+. *P < 0.05.