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Sections through the αHL nanopore (PDB: 7AHL). (A) Charge distribution in the WT αHL nanopore. Positively charged amino acids of αHL are colored in blue and negatively charged amino acids in red. (B) Sites in the αHL nanopore modified in this work. Lys-8 is in purple. The constriction formed by the ion pair Glu-111/Lys-147 is colored in green. Met-113, Thr-115, Thr-117, Gly-119, Asn-121, Asn-123 and Thr-125 are in orange.
