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. 2008 Dec 5;105(50):19720–19725. doi: 10.1073/pnas.0808296105

Fig. 2.

Fig. 2.

Interactions of DNA with the αHL pore at +120 mV. (A) Type A event: A simple low-amplitude event (interpreted as the direct translocation of DNA through the pore). Type B event: A simple midamplitude event (DNA is captured in the vestibule and exits the pore from the side of entry, the cis side). Type C event: A midamplitude signal, followed by a low-amplitude signal within the same event (DNA is captured in the vestibule before it translocates through the β barrel to exit at the trans side). Type D event: A low-amplitude signal, followed by a midamplitude signal within the same event (DNA enters the β barrel directly and then retracts into the vestibule to exit the pore from the cis side). Type E event: A midamplitude signal, followed by a low-amplitude signal, followed by a second midamplitude signal within the same event (DNA explores the vestibule, threads into the β barrel, but retracts back into the vestibule and exits the pore from cis side). (B) Events histogram showing mean residual currents for 2,500 events expressed as fractions of the open pore current through the WT αHL pore. The peaks representing type A and type B events are labeled. The type C, D, and E events, characterized by their mean event currents, appear between the two major peaks. (C) Histogram of the DNA translocation times for the type A events. DNA (0.95 μM) was presented from the cis chamber in 1 M KCl, 100 μM EDTA, 25 mM Tris·HCl (pH 8.0).