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The effects of charged side-chains on the frequency of DNA translocation at +120 mV. (A) M113R-WT (0.46 μM DNA, cis). (B) E111N-WT (0.47 μM). (C) WT (0.53 μM). (D) M113R-RL2 (0.74 μM). (E) RL2 (0.60 μM). (F) M113D-RL2 (2.0 μM). M113D-RL2 showed no current blockades at up to +300 mV. Additional conditions are described in the legend to Fig. 2.
