Table 1.
DNA translocation through the αHL nanopore at +120 mV
| αHL pore | τ, ms | f, s−1·μM−1 | IB | I+120mV, pA |
|---|---|---|---|---|
| WT | 0.141 ± 0.004 (n = 6) | 3.0 ± 0.2 (n = 12) | 0.11 (n = 7) | 125 ± 1 (n = 23) |
| RL2 | 0.147 ± 0.003 (n = 4) | 0.28 ± 0.04 (n = 5) | 0.10 (n = 4) | 124 ± 2 (n = 16) |
| A8R-RL2 | 0.15 ± 0.01 (n = 7) | 7.6 ± 0.7 (n = 5) | 0.10 (n = 7) | 125 ± 3 (n = 10) |
| A8K-RL2 | 0.16 ± 0.03 (n = 3) | 3.0 ± 0.3 (n = 3) | 0.11 (n = 3) | 123 ± 3 (n = 7) |
| M113R-WT | 0.138 ± 0.003 (n = 5) | 23 ± 2 (n = 9) | 0.04 (n = 5) | 140 ± 2 (n = 13) |
| E111N-WT | 0.28 ± 0.04 (n = 10) | 18 ± 1 (n = 9) | 0.11 (n = 10) | 137 ± 3 (n = 15) |
| M113K-RL2 | 0.092 ± 0.002 (n = 3) | 4.0 ± 0.3 (n = 3) | 0.05 (n = 3) | 129 ± 2 (n = 10) |
| M113R-RL2 | 0.141 ± 0.004 (n = 4) | 4.4 ± 0.6 (n = 7) | 0.03 (n = 5) | 131 ± 2 (n = 18) |
| T115R-RL2 | 0.23 ± 0.02 (n = 3) | 2.2 ± 0.1 (n = 7) | 0.02 (n = 3) | 122 ± 2 (n = 16) |
| T117R-RL2 | 0.35 ± 0.04 (n = 8) | 1.6 ± 0.1 (n = 7) | 0.03 (n = 4) | 113 ± 6 (n = 9) |
| G119R-RL2 | 0.292 ± 0.002 (n = 4) | 1.3 ± 0.1 (n = 7) | 0.02 (n = 4) | 111 ± 1 (n = 8) |
| N121R-RL2 | 0.30 ± 0.01 (n = 9) | 0.43 ± 0.03 (n = 10) | 0.03 (n = 6) | 96 ± 2 (n = 13) |
| N123R-RL2 | 0.22 ± 0.02 (n = 5) | 0.43 ± 0.04 (n = 8) | 0.03 (n = 4) | 77 ± 2 (n = 10) |
| M113D-RL2 | Not observed (n = 3) | Not observed (n = 3) | Not observed (n = 3) | 112 ± 3 (n = 3) |
τ is the most probable translocation time; f is the frequency of translocation (type A and C events) normalized to 1 μM DNA; IB is the fractional residual current during a type A event and I+120mV is the open-pore current at +120mV. The errors are expressed as the standard error of the mean and the number of experiments is in parentheses. For IB, the errors were all within ±0.01.