Fig. 2.

ZFN targets in the coil and pask genes. Both strands are shown in the top lines for each target, and the triplets to which ZFs were designed are in bold, red type. Points of expected cleavage on each strand are indicated with arrowheads. The start codon for the long form of coilin is underlined. Mutations recovered from the offspring of injected parents are shown in the context of the top strand. Dashes indicate deleted residues. Substitutions and insertions are shown in bold, blue type. The first coil mutant was recovered twice independently. The 4-bp insertion in the bottom pask mutant is a fill-in and blunt join of the 4-base overhang created by ZFN cleavage.