Fig. 3.

Scheme for isolation of ZFN-induced mutations in the coil gene. mRNAs for the designed ZFNs were injected into wild-type embryos. When adults eclosed they were crossed to parents carrying a deficiency (Df) that includes the coil gene on the second chromosome and a balancer with a dominant marker (CyO). Candidate chromosomes (*) carried over the balancer were isolated individually by crossing again to the same strain. From the second cross, and in some cases from the first cross, individual alleles carried over the deficiency were subjected to molecular analysis.