Fig. 5.

Therapeutic activity of Hph-1-ctCTLA-4 in the collagen-induced arthritis model is mediated by inhibition of T cell activation. (A) The absolute number of CD3- and CD69-positive cells from spleen was analyzed by flow cytometry. (B) CII antigen-specific T cell proliferation was analyzed by thymidine incorporation assay in CIA-induced mice treated with the different protein. Bars represent mean ± SEM of three independent experiments from four mice per group. *, P < 0.05; **, P < 0.01 compared with the control CIA group.