Fig. 5.
Utility of iLOV as a cytosolic fluorescent reporter for TMV infection. (A) Half-leaf inoculations of Nicotiana tabacum show that systemic spread of TMV.iLOV is extensive 4 days post inoculation (dpi) whereas TMV.GFP is still restricted to primary lesions on the inoculated leaf. Arrows indicate half-leaf inoculation sites. (B) Size of TMV.GFP lesion at 2 dpi. (Scale bar, 500 μm.) Lesions typically measured 687 μm ± 103 (n = 14). (C) Size of TMV.iLOV lesion at 2 dpi. (Scale bar, 500 μm.) Lesions typically measured over 3045 μm ± 113 (n = 8).