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. 2008 Dec 5;105(50):20038–20043. doi: 10.1073/pnas.0807551105

Fig. 6.

Fig. 6.

Utility of iLOV as a fluorescent reporter fusion for TMV and PMTV infection. (A) TMV MP-iLOV localization to plasmodesmata. (Scale bar, 20 μm.) (B) Callose staining at plasmodesmata with aniline blue. (C) Colocalization of TMV MP-iLOV fluorescence with aniline blue staining of plasmodesmata. (D) Systemic spread of TMV MP-iLOV and TMV MP-GFP. Upper leaves of Nicotiana tabacum at 4 days post inoculation. TMV MP-iLOV shows extensive systemic spread and unloads from all major vein classes, spreading into neighboring ground tissue (left). TMV MP-GFP by comparison shows no or limited systemic spread unloading only patchily from the midrib and some secondary veins. Leaves were photographed simultaneously to allow direct comparison of green fluorescence intensity. (E) Representative image showing the lesions size produced by PMTV expressing CPRT-YFP 2 days post bombardment of Nicotiana tabacum leaves. (Scale bar, 100 μm.) (F) Lesion size for PMTV expressing CPRT-iLOV visualized as in (E). (Scale bar, 100 μm.)