Fig. 3.

Srx reduces hyperoxidized 2-Cys Prxs in P+ cells. (A) P+ cells were stably transfected with a nontarget ShRNA (ShNT) or an ShRNA construct containing a hairpin structure specific to Srx coding region (ShSrx). Cells were treated with DMSO (−) or TPA (+) for 6 h and Western blotting was performed using whole-cell lysates. (B) Plasmid-expressing Myc-tagged Prx was transfected into P+ cells and immunoprecipitation (IP) was performed with anti-Myc antibody. Western blotting was performed using anti-Myc and anti-Prx-SO3 antibody. (C) Cells stably expressing ShNT or ShSrx were treated with H₂O₂ and then blotted for hyperoxidized Prxs. (D) As in (C), but cells stably expressing empty vector or Flag-tagged Srx.