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Knockdown of Srx impairs AP-1 activation. (A and B). Cells after serum starvation were treated with DMEM containing 50 ng/ml EGF (A) or 5% FBS (B) for indicated time and Western blotting was performed. (C and D). Assay of 4XAP-1 luciferase reporter activity in Srx knockdown cells (C) or in cells co-expressed with Srx (D). Assays were performed in triplicate and data are presented as x ± SD. **, P < 0.05 (t test).
