FIGURE 2.

Restoration of Mitf isoform expression in Mitf^−/− BMMCs. Mitf^−/− BMMCs were transduced with modified pMMP retroviruses that express the mast cell isoform (Mitf-mc), the e-isoform (Mitf-e), and the a-isoform (Mitf-a) (e-isoform). Transduced cells were stably selected in puromycin. The Western blot analysis shows protein expression of each of the individual Mitf isoforms and the vector control. Protein expression in wild-type (Mitf^+/+) and Mitf^−/− BMMCs are also shown. The Western blot for anti-tubulin (α tubulin) expression demonstrated comparable loading of samples.