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. Author manuscript; available in PMC: 2008 Dec 17.
Published in final edited form as: J Immunol. 2007 Jan 1;178(1):378–388. doi: 10.4049/jimmunol.178.1.378

FIGURE 4.

FIGURE 4

Mitf isoforms restore SCF-directed migration in Mitf−/− cells. BMMCs were placed in Transwell chambers coated with fibronectin or BSA as a control (described in Material and Methods). Chambers were placed in medium with or without SCF. Migration and binding to fibronectin was quantified by counting adherent cells on an inverted microscope at X40 and indicated on y-axis by cells migrated per HPF (high-powered field). In wild-type cells (Mitf+/+), binding to fibronectin was dependent on SCF treatment (□); in Mitf−/− cells, binding was severely impaired. Expression of the mast cell isoform (Mitf-mc), the e-isoform (Mitf-e), and the a-isoform (Mitf-a) restored SCF-dependent binding. Data are expressed as the mean result of experiments performed in triplicate. Error bars indicate SD.

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