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successive steps of the missing peak chromatography applied to the screening of nicotinic ligands in the tobacco smoke condensate.
| Process step | Operation |
|---|---|
| 1 | Extraction and fractionation to yield Fraction 1 |
| 2 | (S)-nicotine depletion to yield Fraction 1A |
| 3 | Chromatographic fingerprint of Fraction 1A |
| 4 | Chromatography of Fraction 1A on nAChR(+)-CMAC and nAChR(−)-CMAC |
| 5 | Determination of chromatographic fingerprints of collected fractions |
| 6 | Comparison of chromatographic fingerprints |
