Table 2.

The compounds identified in Fraction 1A of the tobacco smoke condensate using retention times (Rt) and molecular ions ([M+H]⁺) and their relative affinities for the immobilized α₃β₄ nAChR determined by the reduction in the retention volume of [³H]-epibatidine (Δml) produced by the addition of a 10 µM concentration of the compound to the mobile phase.

Compounds	Rt (min)	[M+H]+	Δml
(S)-Nicotine	17.5	163.2	40
(R,S)-Anatabine	20.6	161.2	42
N’-Nitrosonornicotine	29.0	178.2	42
(R,S)-Nornicotine	14.5	149.2	36
Anabasine	23.1	163.2	32
N-Methyl-γ-oxo-3-pyridinebutanamide	19.0	193.4	18
(1’S,2′S)-Nicotine 1’-oxide	21.8	179.2	4